Basic Training Module

Microbiology Techniques – Basic

Basic Module 1

Duration: 7 Days

Training fees: ₹1500/-

Course Content

• Basics of Lab Safety: Lab safety and Procedures, Scope & Applications, Record Maintenance, Handling of Equipment

• Overview and types of Sterilization techniques, Concept of Solution Chemistry and Concentration, Keeping & storage of lab chemicals, reagents & solvents

• Type of Media, Media Preparation (NAM, EMB, MSA, XLD, BHI etc.,)

• Sample collection and Serial Dilution technique

• Culture techniques (pour plate, spread plate and streak plates methods)

• Pure culture preparation (bacteria and fungi)

• Gram’s staining, Endospore staining, Lactophenol aniline cotton blue staining.

• Microscopy, correct methods of handling microscope.

Biochemistry Techniques – Basic

Basic Module 2

Duration: 7 Days

Training fees: ₹ 2000/-

Course Content

• Concept of Biochemistry, Scope & Applications.

• Basics of Lab Safety: Lab safety and Procedures, Record Maintenance, Handling of Equipment

• Quantitative estimation of Proteins by Lowry’s method (Spectrophotometric)

• Estimation of Total carbohydrates by Anthron or DNS method (Spectrophotometric)

• Estimation of Total fats

• Estimation of Saponification value of Fats or Oils

• Estimation of Ascorbic acid in food or beverage samples

• Thin Layer Chromatography analysis of extracts for qualitative detection of marker compounds (phenolic, flavonoid or amino acid).

Molecular Biology Techniques – Basic Genomic Techniques

Basic Module 3

Duration: 7 Days

Training fees: ₹5000/-

Course Content

• Basics of Lab Safety: Lab safety and Procedures, Scope & Applications, Record Maintenance, Handling of Equipment

• Extraction of DNA from Plant samples by CTAB method

• Extraction of Bacterial DNA by PCI method

• Qualitative and quantitative analysis of extracted DNA by Electrophoresis on 2% agarose gel and by Spectrophotometry.

• PCR Techniques and protocol for extracted DNA samples 

• Agarose gel electrophoresis of PCR product & gel documentation for detection amplified DNA fragment.

Molecular Biology Techniques – Basic Proteomics and SDS-PAGE Techniques

Basic Module 4

Duration: 7 Days

Training fees: ₹5000/-

Course Content

• Basics of Lab Safety: Lab safety and procedures for protein handling, Scope & Applications, Use of PPE, chemical safety (acrylamide toxicity awareness), Record maintenance, Handling of electrophoresis units & power supplies.

• Protein Extraction from Biological Samples: Extraction of total soluble proteins from plant/animal/microbial samples, Use of extraction buffers (Tris-HCl, β-mercaptoethanol, SDS), Protease inhibitors & sample preservation

• Qualitative and Quantitative Estimation of Proteins: Bradford assay by Spectrophotometric method, Preparation and use of standard curve (BSA), Importance of uniform loading

• Preparation of SDS-PAGE Gel

1. 1. • Composition of resolving & stacking gels
      • Preparation of polyacrylamide solutions
      • Gel casting, polymerization, and assembly
      • Sample preparation with Laemmli buffer (denaturation)

• Running SDS-PAGE

1. 1. • Loading samples & protein ladder
      • Electrophoresis conditions (voltage, buffer, timing)
      • Troubleshooting: smiling bands, distorted bands, leakage

• Staining & Destaining of Protein Gels

1. 1. • Coomassie Brilliant Blue staining
      • Destaining for band visualization
      • Introduction to silver staining (optional advanced introduction)

• Gel Documentation & Analysis

1. • Imaging of stained gels
   • Estimation of molecular weight using marker proteins
   • Interpretation of band intensity and pattern
   • Basic protein profiling applications

Food Biotechnology: Quality Analysis of Food, Milk and Beverages

Basic Module 5

Duration: 7 Days

Training fees: ₹ 2500/-

Course Content

• Concept of Food Biotechnology and Microbiology, Scope & Applications

• Basics of Lab Safety: Lab safety and Procedures, Record Maintenance, Handling of Equipment

• Sampling techniques for quality analysis of solid and liquid food or beverage

• Microbiological evaluation by total bacterial and total fungal enumeration in sample

• Detection of Coliforms on selective media

• Detection of yeast cell in food & beverages

• Milk quality & adulteration test by;

1. 1. 1. Microbiological quality check MBRT Test
      2. COB Test & Alcohol Test
      3. Detection of starch
      4. Detection of water
      5. Detection of formalin
      6. Detection of soap 
      7. Detection of detergent

• General quality analysis of fruit juices: 

1. 1. pH analysis
   2. visual & sensory analysis
   3. Total soluble solids by refractometer

Medical Biotech/Microbiology: Antibiotic Sensitivity Assay Methods

Basic Module 6

Duration: 10 – 15 Days

Training fees: ₹ 3000/-

Course Content

• Basics of Lab Safety: Lab safety and Procedures, Scope & Applications, Record Maintenance, Handling of Equipment

• Overview and types of Sterilization techniques, Concept of Solution Chemistry and Concentration, Keeping & storage of lab chemicals, reagents & solvents

• Concept of Antimicrobial Agents: Types of antimicrobial compounds, Antibiotics vs. antifungals vs. herbal extracts and Bactericidal vs. bacteriostatic action

• Media & Reagent Preparation for AST

1. 1. 1. Pre-experimental preparations
      2. Mueller Hinton Agar (MHA) preparation
      3. Nutrient Broth, Nutrient Agar
      4. Sterilization and storage of media, discs, and filters

• Preparation of Test Microorganisms

1. 1. 1. Revival and maintenance of bacterial cultures
      2. Preparation of inoculum (0.5 McFarland Standard)
      3. Spread plating for lawn culture

• Antibiotic Sensitivity Testing Methods: Kirby–Bauer Disc Diffusion Technique, Agar Well Diffusion Method, Determination of Zone of Inhibition

• Minimum Inhibitory Concentration (MIC) – Basics: Broth dilution overview, Serial dilution of antimicrobial agents/extracts, Observation of turbidity and interpretation

• Hands-on with Antibiotic Discs & Herbal Extracts

1. 1. 1. Placing and labelling antibiotic discs
      2. Loading wells with extracts
      3. Incubation, observation, zone diameter measurement

• Clinical Sample Testing

1. 1. 1. Overview of blood, urine, pus, wound samples cultures
      2. Practical handling and antibiotic sensitivity assay with standard antibiotics

• Result Interpretation

1. 1. Measuring zones and reporting using standard tables
   2. CLSI standard interpretation (Sensitive / Intermediate / Resistant)
   3. Report writing and data representation (tables, graphs)

Pharmaceutical Biotechnology: Phyto Drug Extraction Technology – Basic

Basic Module 7

Duration: 7 Days

Training fees: ₹ 2000/-

Course Content

• Concept of Phytodrug and Pharmaceutical Biotechnology, Scope & Applications.

• Basics of Lab Safety: Lab safety and Procedures, Record Maintenance, Handling of Equipment

• Collection of plant material for drug extraction

• Extraction of phytochemicals by maceration, infusion and Soxhletion methods

• Concentration of Phytochemical Extracts and organoleptic assessment

• Preliminary qualitative test to detect different bioactive chemical constituents;

1. 1. 1. Alkaloid test
      2. Flavonoid test
      3. Tannin test
      4. Terpenoid test
      5. Glycoside test
      6. Saponin test

• Thin Layer Chromatography analysis of extracts for qualitative detection of marker compounds (quercetin or gallic acid).

Industrial Biotechnology: Enzyme Production

Basic Module 8

Duration: 7 Days

Training fees: ₹ 3500/-

Course Content

• Concept of Industrial Biotechnology, Scope & Applications – Enzymes, Biofuels, Bioplastics, Organic Acids, Fermented Products.

• Basics of Lab Safety: Lab safety and Procedures, GLP/GMP basics, Record Maintenance, Handling of Instruments and Industrial Microbes.

• Media Preparation for Industrial Bacteria; LB, NB, and minimal salt media.

• Fermentation Techniques: Introduction to batch, fed-batch & continuous fermentation.

• Experimental Work: Shake flask fermentation for amylase enzyme production by B. megaterium or B. subtilis.

• Extraction of Crude Enzyme 

• Enzyme Assay; Qualitative and quantitative enzyme assay by spectrophotometry.

• Industrial Documentation; Batch record preparation, SOP writing, QC/QA basics, report preparation.

Industrial Biotechnology: Enzyme Production

Basic Module 9

Duration: 15-20 Days

Training fees: ₹ 5000/-

Course Content

• Concept of Industrial Biotechnology, Scope & Applications – Enzymes, Biofuels, Bioplastics, Organic Acids, Fermented Products.

• Basics of Lab Safety: Lab safety and Procedures, GLP/GMP basics, Record Maintenance, Handling of Instruments and Industrial Microbes.

• Media Preparation for Industrial Microbes: For bacteria, yeast & fungi (YPD, Czapek Dox, PDB, NB, minimal salt media).

• Fermentation Techniques: Introduction to batch, fed-batch & continuous fermentation. Experimental work on Shake flask fermentation for enzyme or alcohol.

• Enzyme Extraction & Assay; Extraction of crude enzymes (amylase/cellulase/protease). Qualitative and quantitative enzyme assay by spectrophotometry.

• Downstream Processing: Filtration method, centrifugation method, and precipitation method (salt or solvent based), crude protein concentration.

• Bioproduct Development: Demonstration of microbial product such as:

• Ethanol / Vinegar / Pigment production OR
• Organic acid (citric/lactic) detection

• Quality Analysis: pH, enzyme activity by total estimation of total reducing sugars or glucose release, total protein estimation, product confirmation by UV-Vis or TLC

• Industrial Documentation: Batch record preparation, SOP writing, QC/QA basics, report preparation.

Industrial Biotechnology: Organic Acid Production

Basic Module 10

Duration: 7 Days

Training fees: ₹ 3500/-

Course Content

• Concept of Organic Acids, Scope & Applications – Enzymes, Biofuels, Bioplastics, Organic Acids, Fermented Products.

1. 1. • Citric acid, lactic acid, acetic acid, gluconic acid
      • Uses in food, pharma, beverages, bio-preservation & industrial bioprocessing

• Basics of Lab Safety: Lab safety and Procedures, GLP/GMP basics, Record Maintenance, Handling of Instruments and Industrial Microbes.

• Media Preparation for Organic Acid–producing Microorganisms

1. 1. • PDA, YPD, Czapek-Dox media for Aspergillus niger (citric acid)
      • Glucose Yeast Extract Calcium Carbonate (GYC) media & broth for Acetobacter aceti (acetic acid).

• Fermentation Techniques & Parameters: Introduction to batch, fed-batch & continuous fermentation. Experimental work on Shake flask fermentation for organic acid production.

• Experimental Work

1. 1. • Shake flask fermentation for citric acid production using Aspergillus niger*
        OR
      • Acetic acid production using Acetobacter species

• Extraction & Purification of Organic Acids: Filtration, Acid precipitation, Solvent extraction (demonstration) and Crude acid concentration

• Quality Analysis of Organic Acids: pH measurement and TLC for identification of organic acids and UV–Visible spectrophotometric analysis.

• Industrial Documentation: Batch record preparation, SOP writing, QC/QA basics, report preparation.

Agriculture Biotechnology – Biofertilizer Production & Quality Control

Basic Module 11

Duration: 7 Days

Number of seats per batch: 10 only

Training fees: ₹ 3500/-

Course Content

1. Introduction to Agricultural Biotechnology & Biofertilizers

• Types of biofertilizers: Rhizobium, Azotobacter, Azospirillum, PSB (Phosphate Solubilizing Bacteria), KSB (Potassium Solubilizing Bacteria), Cyanobacteria (Anabaena, Nostoc) and Trichoderma/Biocontrol agents (optional)
• Advantages over chemical fertilizers
• Agricultural applications & market demand

2. Basics of Lab Safety: Biosafety Levels (BSL-1 organisms), PPE, waste disposal, autoclaving, Culture handling and sterilization, GLP basics and record keeping
3. Media Preparation for Biofertilizers: Preparation of production media: Yeast Extract Mannitol Agar (YEMA) – Rhizobium and Jensen’s N-free medium – Azotobacter
4. Isolation and Culturing of Biofertilizer Strains

• Isolation of bacteria from soil/root nodules
• Streaking, spreading, subculturing
• Colony observation, pigmentation, morphology
• Selective media identification

5. Mass Production of Biofertilizers: Inoculum preparation in broth, Shake flask fermentation, Carrier preparation & Culture mixing, Packaging of biofertilizer powders/slurry
6. Quality Control Tests of Biofertilizers: Cell count (CFU/mL or g), Contamination test, pH measurement, Moisture content, Viability test, Carrier quality testing, Shelf-life monitoring
7. Documentation & Industrial Compliance: Batch production record, Labelling requirements, NABL / FCO / Biofertilizer standards in India, SOP writing & report preparation

Agriculture Biotechnology – Biofertilizer Production & Quality Control

Basic Module 12

Duration: 15-20 Days

Number of seats per batch: 10 only

Training fees: ₹ 5500/-

Course Content

1. Introduction to Agricultural Biotechnology & Biofertilizers

• Types of biofertilizers: Rhizobium, Azotobacter, Azospirillum, PSB (Phosphate Solubilizing Bacteria), KSB (Potassium Solubilizing Bacteria), Cyanobacteria (Anabaena, Nostoc) and Trichoderma/Biocontrol agents (optional)
• Advantages over chemical fertilizers
• Agricultural applications & market demand

2. Basics of Lab Safety: Biosafety Levels (BSL-1 organisms), PPE, waste disposal, autoclaving, Culture handling and sterilization, GLP basics and record keeping
3. Media Preparation for Biofertilizers:

• Preparation of production media:
  • Yeast Extract Mannitol Agar (YEMA) – Rhizobium
  • Jensen’s N-free medium – Azotobacter
  • Okon medium – Azospirillum
  • Pikovskaya’s medium – PSB
• Sterility testing and contamination control

4. Isolation and Culturing of Biofertilizer Strains

• Isolation of bacteria from soil/root nodules
• Streaking, spreading, subculturing
• Colony observation, pigmentation, morphology
• Selective media identification

5. Mass Production of Biofertilizers

• Inoculum preparation in broth
• Shake flask fermentation
• Carrier preparation
  • Lignite/peat/charcoal/talc-based carriers
• Mixing culture with carrier
• Packaging of biofertilizer powders/slurry

6. Quality Control Tests of Biofertilizers: Cell count (CFU/mL or g), Contamination test, pH measurement, Moisture content, Viability test, Carrier quality testing, Shelf-life monitoring

• Efficiency test on plates:
  • Rhizobium: Congo red/YEMA reaction
  • PSB: Zone of clearance on Pikovskaya agar
  • Azotobacter: Pellicle formation, pigment
  • Azospirillum: White pellicle microaerophilic growth

7. Application Methods in Agriculture: Seed treatment, Seedling root dip, Soil application, Compatibility with fertilizers & pesticides, Field-level precautions
8. Documentation & Industrial Compliance

• Batch production record
• Labelling requirements
• NABL / FCO / Biofertilizer standards in India
• SOP writing & report preparation

Plant Biotechnology: Plant Disease Investigation – Basic

Basic Module 13

Duration: 7-10 Days

Training fees: ₹ 3500/-

Course Content

• Basics of Lab Safety: Lab safety and Procedures, Scope & Applications, Record Maintenance, Handling of Equipment

• Overview and types of Sterilization techniques, Concept of Solution Chemistry and Concentration, Keeping & storage of lab chemicals, reagents & solvents.

• Introduction to Plant Diseases

• Types of plant pathogens: fungi, bacteria, viruses, nematodes
• Field symptoms: leaf spot, blight, wilt, rot, canker, mosaic
• Importance of disease diagnosis in agriculture and research

1. Field Sample Collection
   • How to select diseased plant parts
   • Tools for sampling (scalpel, forceps, paper bags, vials)
   • Labelling, transport & storage of samples
   • Avoiding cross-contamination
2. Preparation of Media for Pathogen Isolation: Introduction to common media: PDA, NA, nutrient broth and their preparation, Plate labelling and storage
3. Isolation of Plant Pathogenic Fungi
   • Surface sterilization of diseased tissue
   • Tissue segment plating method
   • Spore suspension plating
   • Incubation and observation of colony morphology
4. Isolation of Plant Pathogenic Bacteria
   • Streaking & spread plate methods
   • Colony selection from diseased plant exudates
   • Pure culture preparation
5. Microscopic Examination
   • Slide preparation for fungi and bacteria
   • Lactophenol cotton blue staining for fungal spores
   • Gram staining for bacteria
   • Identification based on shape, hyphae, spore structures
6. Koch’s Postulates (Basic Demonstration)
   • Inoculation of healthy plant tissue
   • Observation of symptoms
   • Re-isolation to confirm pathogenicity
7. Molecular & Rapid Detection (Introduction and Discussion only): Basics of PCR and ELISA for plant pathogens, Use of immuno-strip and lateral flow kits.
8. Result Interpretation & Reporting
   • Recording culture observations
   • Disease identification sheet preparation
   • Photography, documentation, and lab notebook maintenance

Animal Biotechnology: Animal Tissue Culture Technique – Basic

Basic Module 14

Duration: 7 Days

Number of seats per batch: 10 only

Training fees: ₹ 10000/-

Course Content

• Concept of Animal Cell & Tissue Culture, Scope & Applications.

• Basics of Lab Safety: Overview of cell culture laboratory, Lab safety and Procedures, Record maintenance, Equipment used in cell culture lab

• Aseptic handling techniques for handling cell lines and related procedures

• Detection of contaminants in cell culture and control measures

• Types of animal cells and their characteristics in culture

• Preparation of cell culture media and their sterilization methods

• Revival of cells from Liquid nitrogen (cell bank)

• Sub-culturing or passaging of cells

• Inverted microscopy of cultured cells, Cell counting and cell viability test

• Cryopreservation of cell lines

Animal Biotechnology: Rearing & Handling of Drosophila Model – Basic

Basic Module 15

Duration: 15-20 Days

Number of seats per batch: 10 only

Training fees: ₹ 10000/-

Course Content

• Basics of Lab Safety: Overview of Animal biosafety guidelines and ethical considerations, PPE usage, sterile handling, disinfection, Lab safety and Procedures, Record maintenance & experimental documentation, Equipment, Handling glassware, CO₂ anaesthesia used in Fly Lab.

• Introduction to Drosophila as a Model Organism

1. • Why Drosophila is used in genetics, toxicology, developmental biology
   • Life cycle: egg → larva → pupa → adult
   • Male vs female identification
   • Strains and wild-type vs mutant types

• Preparation of Drosophila Culture Media

• Ingredients of standard media, Cooking, autoclaving & dispensing media
• Storage & shelf-life of media

• Rearing & Maintenance of Fly Cultures

• Setting up stock cultures
• Maintaining humidity, temperature & light cycle
• Preventing contamination & mite infection
• Transfer of flies to fresh media (sub-culturing)

• Handling and Anesthetizing Flies

• CO₂ anesthesia setup / cold anesthesia, Safe handling to avoid injury and 
• Sorting of male & female flies
• Counting and transferring flies to vials

• Life Cycle Observation

• Egg collection, larval stages, pupation and eclosion
• Microscopic observation of larvae and adult structures, Recording developmental time at different temperatures

• Basic Experiments (Demonstration)

• Mating experiments
• Observation of phenotypic mutants
• Longevity / behavior studies (basic introduction)
• Simple toxicology or dietary exposure experiment (optional)

• Disposal, Hygiene & Record Keeping

• Proper killing and disposal of flies, cleaning of vials and lab space
• Maintaining stock label, date, strain name
• Data recording and documentation